6 mm). 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. . All of the experiments were performed in triplicate, and the results were represented as mean. 1. Introduction. 5. MTT values at day 3 were normalized to day 0. Phillips5, Martin A. The medium was then removed from the wells, and 100 µl of DMSO was added to solubilize the formazan crystals. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Shop our huge selection of OEM, MRO, construction, industrial, and safety products. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. free. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Colony formation assays were performed to measure cell proliferation. 2. NCI-60 Human Tumor Cell Line Screen. Background-subtracted data values (mean +/- SD) are graphed. SpectraMax® Paradigm® Multi-Mode Detection Platform. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). The following document is intended to demonstrate setup of this instrument. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeThen, cells were stained with MitoSox Red (5 μM) for 30 min. 2. org) with optimized instrument settings (Table 1) were used to generate and analyze data. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Background-subtracted data values (mean +/- SD) are graphed. To avoid injury, it is recommended that two. D 1 1. Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). 2. . Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. • Tel. Detection modalities are shown in Table 1-1. Statistical. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Recertification is recommended every two years. Inhibition of CPT-11 Hydrolysis by Four Bioflavonesfor the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. SPECTROMAXx with iCAL 2. Fluorescence intensities of each well were examined by a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) with 485-nm excitation and 525-nm emission wavelengths. 5. 1. Parameter SpectraMax iD5 SpectraMax i3x. Microplate Readers include the M3, M4,. Polybead® nanoparticles were analyzed. After aerosol collection was completed, the impactor plates were rinsed with 0. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. SpectraMax M2/M2e reader: TRUE: Molecular Devices: Aquamax DW4 washer: TRUE: Molecular Devices: SpectraMax M4 reader: TRUE: Molecular Devices: SpectraMax M5/M5e reader: TRUE: Molecular Devices:. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. When a feature or capability applies to only certain readers, this exception is noted. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. Bioz Stars score: 86/100, based on 1 PubMed citations. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. (Shanghai, China). Choose cables that meet the requirement described in this appendix. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 1 Figure from 528 Publications Molecular Devices LLC. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. For the quantification of the number of viable bacteria in the biofilm, the biofilms were gently destroyed and plated on TSA after serial. Data analysis methods. Images were taken using a fluorescence microscope (Nikon 80i, Japan). 03 in x 3. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. Cellular Reactive Oxygen Species (ROS) was measured in Neuro2a cells seeded in 6-well plate at a density of 5000 cells/well. . Record fluorescence emission data in kinetic mode for 2 min with 20 s between reads to ensure that interaction has reached equilibrium and take average. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. In the M2, M5 and Plus 384, the light. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. SpectraMax® ABS. 5 mL of creatinine, FD-4, and rhodamine70 dissolved in. A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. Briefly, 4T1 cells were seeded (5000 cells/well) in a 24-well plate. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. Table 2. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). F1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Background-subtracted data values (mean +/- SD, n =2) are graphed. SpectraMax® M5. StakMax Handling Stacker Base Unit. ISM Equipment Classification (Group 1, Class A) This equipment is. Cet appareil ISM est confomre à la norme NMB-001 du Canada. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-Mode MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e. 8. 3. 9000-0756. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. 4. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. , cyan fluorescence at 458 ex. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. Fluoresbrite® fluorescent nanoparticles were analyzed by spectrofluorimetry where particles were excited at 441 nm wavelengths and emission intensity was recorded at 486 nm wavelengths. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. CMC values were determined from the pyrene fluorescence in solution as a function of conjugate. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. 4) and cells were stained with 150 nM TSQ for 1 min. S. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Lift up the lid over the chamber to insert or remove a cuvette. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. AM (~0. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). . Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1, FlexStation 3 reader, SpectraMax L reader, LMax II (Obsolete), SpectraMax M5/M5e reader. Table 2. 314 low lamp level warning. 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 161 likes. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. High tolerance for contaminants. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. SpectraMax® M4). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The innovative and flexible design features. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. However, traditional polyacrylamide gel electrophoresis (PAGE) and high. SpectraMax Microplate Readers. 次いで、ユーザーのラボに. 383 inches whereas the maximum allowed is 5. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Roelli1, Amandine Stoss1, Rebecca A. WARNING! The instrument weighs approximately 36 pounds (16. 1. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. Moving a SpectraMax Multi-Mode Microplate Reader. ® reader to the computer. Lastly, the TMB substrate (Beyotime) was added, and absorbance was measured at 450 nm by a microplate reader (SpectraMax M4). Viability was determined by recording absorbance at 570 nm with plate reader (SpectraMax M4 Multimode, USA) and cell viability was determined. 90-0083 series): Excitation 530 nm,. 4. For cell cultures, the medium was removed 24 h after initiation of drug stimulation and frozen for subsequent analysis, with no need for sample dilution. One of the. Example: [Drawer]. Cellular viability was assessed by using resazurin at 1. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Titration of human serum and plasma (heparin) within the working range of the assay. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. There are multiple vendors. All luciferase assays were conducted in CORNING ® TypeI Collagen Coated 96-Well Cell Culture Clear Bottom White plates. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Description 1. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. The DQ Green BSA probe. 306 plate air cal fail,low light. All experiments were performed in triplicate. 3, La Jolla, CA, USA). Highly selective for dsDNA over ssDNA or RNA. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal)0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Compare Fluorescence Microplate Readers from leading suppliers on Biocompare. Description 1. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Microscopic evaluation of apoptosis. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. SpectraMax M Series Multi-Mode Microplate Readers | Molecular Devices · 2020-04-14 · use two scanning monochromators so the user can determine optimal excitation and emission settings, of 8 /8 Match case Limit results 1 per pageThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). 2. Bio-layer interferometry assays The binding kinetics of 1H1 to spike or RBD proteins from SARS-CoV-2 variants was performed by biolayer interferometry analysis on the GatorPrime Label-Free Bioanalysis. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Table 2. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Standard features include a cuvette port,. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Plot the fluorescence intensity versus the cell seeding number. In. Background-subtracted data values (mean +/- SD) are graphed. The incubation system pre-incubated at 37°C for 3 min minutes and 2 μL fluorescent substrate FD (5 μM, final concentration) was added for fluorescence analysis in fully automated fluorescent microplate reader (SpectraMax M4, Molecular Devices) (Wang et al. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax Spectrofluorometers. Choose cables that meet the requirement described in this appendix. 2. 7°C and the bottom to 36. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SPECTRAMAX 190 reader: TRUE: Molecular Devices: SPECTRAMAX 340PC/340PC 384 reader: TRUE: Molecular Devices. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 3 or greater. Table of Contents. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. TYPICAL SAMPLE VALUES Sensitivity: The calculated minimal detectable dose (MDD) is 15. A microplate reader (SpectraMax M4; Molecular Devices) was used to measure the absorbance (Abs) at 545 mM and the hemolysis rate was calculated as follows: (Eq. SpectraMax® Paradigm® Multi-Mode Detection Platform. SpectraMax® Paradigm® Multi-Mode Detection Platform. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Description 1. Compare plate readers7 Chapter 1: Installation and Setup SoftMax Pro Microplate Data Acquisition and Analysis Software controls Molecular Devices spectrophotometers, absorbance, luminescence, and fluorescence microplate readers, and the SpectraMax Paradigm Multi-Mode Detection Platform. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Quick, Simple Standardization: The new analyzer features SPECTRO’s proprietary iCAL 2. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). 0 statistics software. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. FlexStation ®, SpectraMax. The following document is intended to demonstrate setup of this instrument. Cells were seeded at 5000 cells well −1 on 96-well plates and allowed to grow for 24 h before treatment. Examples: “Press carefully to engage,” “ Do not press down. Subsequently, 20 µl CCK-8 solution (5 mg/ml) diluted in PBS was added to each well for another 1 h and the absorbance was measured at 450 nm using a SpectraMax M4 microplate reader (Molecular Devices, LLC). The optical systems Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. . The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. Devices SpectraMax® M4). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. 2. 1. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. FlexStation™ III. The combination index (CI) was calculated. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. StakMax Handling Stacker Base Unit. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4Relative florescence units (RFU) were quantified on a plate reader (SpectraMax®; M4; Molecular Devices, Sunnyvale, CA) every 1 minute over a 10‐minute period at 37°C, using excitation and emission wavelengths of 328 and 420 nM, respectively. SpectraMax® ABS Plus. Host your own live radio show with. The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). 37. Results were expressed as a percentage relative to the control group (defined as 100%). The IP-10 standard curve was prepared as described in Section 10. Then cells were collected, and the caspase-3 expression level was detected with the caspase-3 assay kit. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The MDD was determined by calculating the mean of zero standard replicates (n=24) and. Ltd. 0397 in x 3. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. SpectraMax® L (software version 5. For the microspheres containing moxifloxacin used in vivo, the liquid hydrogel was presoaked in a 24 mg/mL aqueous solution of moxifloxacin hydrochloride. View specifications, prices, citations, reviews, and more. TEM. 304 signal level saturation. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. D 1 1. The Multi-mode readers are top and bottom read. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Laboratory Freezers. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Results. After 7 days of culture, the colonies generated. 38. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. 6. All configurations. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. In the M2, M5 and Plus. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Our absorbance plate readers feature our PathCheck Sensor. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Detection modalities are shown in Table 1-1. , USA). In vitro transcription (IVT) is an essential technique for RNA synthesis. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. For heme titration experiments a constant ATP concentration of 1 mM was used. +1 250-415-0785. Plus 384 ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Highly selective for dsDNA over ssDNA or RNA. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. The SpectraMax M2 and M2e are versatile and reliable. Bottom-reading is usually recommended for cell-based assay with adherent cells. 2. com. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 1. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 2. Roelli1, Amandine Stoss1, Rebecca A. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. Plate Formats: 6, 12, 24, 48, 96, 384 Well Plates. Fluorescence Microplate Reader Comparison Chart. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. 5 pM 2. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. Flasks containing 100 mL of YM broth were inoculated with 100. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. 1. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. 7. Data are presented as mean values ± standard. Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. The MTT reagent was incubated with cells for 4 h. SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. > spectramax m4 microplate reader. All experiments. . 2. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. The absorbance was determined by a microplate reader (SpectraMax M4, USA) at 405 nm. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Acridine orange (AO) staining evaluates the morphological changes in apoptotic cells by formation of blebs in the membrane, fragmentation and condensation of the. The ability of the liposome to be internalized into cell was assessed using cell culture studies 45-47. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). Choose from a three- (M3), four- (M4), orCables. The plate reader optics are tunable for absorbance. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. VIEW DETAILS. SpectraMax M3, M4, M5, and M5 e. Category 2 and 3 microplate readers use gradient heating as well. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 4) In vivo pharmacokinetic studies. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. The SpectraMax M5e reader offers the additional benefit of being certified . The data represent the mean ± SD of three independent experiments. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal) 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. . The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Louis, MO, USA). GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. e. . The IL-10 standard curve was prepared as described in Section 10. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. . Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. No. Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. These samples were analyzed using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Background-subtracted data values (mean +/- SD) are graphed. 116 SpectraMax M5 and M5e Multi-Mode. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. The IC50 curves were calculated using variable slope for log (inhibitor) vs. 2. Example: [Drawer]. 9000-0756. Other technologies such as imaging. Luminescence was immediately measured by a SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA) after mixing the fecal supernatant and Hgb standard with the same amount of luminol reagent. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Plot the fluorescence intensity versus the cell seeding number. Large Molecule Bioanalysis. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Data are from ≥5 independent experiments. 1 & 7. 2. The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). Click Read.